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Expression levels were decided by a quantitative colorimetric assay [BioAssay Systems Cat # DUCT-500 for creatinine Cat # DIUR-500 for BUN], using an improved Jaffe method for creatinine, and an improved Jung methodology for BUN without any pretreatment of the samples. The number of proliferative cells was decided using the PCNA Staining Kit (Invitrogen, c# 93–1143) at 24 hours, forty eight hours, 72 hours, 1 week and a pair of weeks after injections in each experimental group above described as suggested by the equipment knowledge sheet. Briefly, after deparaffination and rehydration and blocking, the slides have been incubated with a biotinylated mouse anti-PCNA major antibody for 1 hour at room temperature adopted with incubation with Streptevidin-HRP and revealed with DAB substrate. In all the experimental teams the proliferative nuclei were counted as a fraction of the whole variety of nuclei current within the section using consecutive, non-overlapping fields of PCNA-stained specimens. The share of proliferative cells was estimated with out information of the experimental group. At totally different occasions factors , the injected and the control mice had been sacrificed.